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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be described. Two additional splice variants have been claimed but specific nucleotide sequence info just isn't however obtainable.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible success are...
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This information outlines more achievable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as website opposed to the antibody by yourself, one can see which staining is particular.
Goal Details Cyclin E belongs into the extremely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinct expression and degradation styles which add for the temporal coordination of every mitotic party. Cyclin E sorts a fancy with and capabilities like a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates for the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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